Journal of Applied Cosmetology

Skin Quality and Aging: New Frontiers in Hydration, Firmness, and Cellular Regeneration

2025-04-09T11:01:33+00:00

Skin aging involves a progressive decline in hydration, firmness, and regenerative capacity (1). The objective of this study is to evaluate the clinical effects and elucidate the mechanism of action of a novel biostimulator formulation composed of fragmented hyaluronic acid, choline, and essential amino acids on key indicators of skin quality, including hydration, dermal firmness, and cellular regeneration. The study further seeks to position this formulation as a low-inflammatory alternative to conventional aesthetic treatments that rely on controlled tissue damage, contributing to the development of biocompatible, physiologically integrative approaches in regenerative aesthetics. This study seeks to establish new therapeutic frontiers in aesthetic medicine, where biocompatibility, physiological biostimulation, and preservation of the cellular environment become pillars of healthy and conscious aging. Twenty patients aged between 35 and 65 years were treated over a 12-week period. Skin firmness was measured using cutaneous elastometry. Subjective and biometric evaluations were conducted. A 28% improvement in skin firmness was observed through elastometry (p<0.05). Patients reported significant improvements in hydration, tone, and luminosity. Biostimulatory solution for facial rejuvenation has illustrated the effect on physiological changes that mitigate facial aging.

A Comparative Case Study on Echographic Behaviour of Selected Soft Tissue Fillers

2025-05-05T07:13:01+00:00

Soft tissue fillers, whether based on cross-linked hyaluronic acid (HA) or other ingredients like calcium hydroxyapatite (CaHA), have been developed for long-term facial volume restoration. This case study aims to assess the behaviour, efficacy, durability, tolerability, integration, and degradation of selected soft tissue fillers in treating soft tissue deficits. Nine subjects were randomly divided into three groups, each receiving different soft tissue dermal fillers. Symmetrically, 0.5 ml of product was administered per side subcutaneously in the zygomatic arch area using a sterile 22G cannula via puncture with a sterile 21G needle. Patients underwent treatment at baseline (week 0), with no optional touch-ups allowed. Assessments were conducted at month 1, 3, and 6 using the HF-USG imaging. High-frequency ultrasonography (HF-USG) imaging of the zygomatic arch area was performed at all visits. Adverse events (AEs) were monitored and recorded. Analysis of HF-USG images indicated superior properties for PEGDE cross-linked acid, with no reported adverse events. All participants demonstrated aesthetic improvement, with projection and volumetric effects varying among the filler types. The study underlines the importance of filler selection and anatomical knowledge in achieving desired outcomes. High-frequency ultrasound offers valuable insights into filler behaviour and tissue response. Further research with larger sample sizes is warranted to validate these findings and explore additional parameters.

Case Study on a Multi-Layer Approach to Skin Rejuvenation: Effects of Energy-Based Devices and Injectable Biostimulatory Therapies

2025-05-28T10:31:58+00:00

Achieving smooth, healthy-looking skin is a common aesthetic goal across all age groups. This study assessed the effectiveness of a novel protocol combining non-crosslinked hyaluronic acid (HA), hydro-exfoliation, infrared technology and non-ablative fractional 1470 nm laser to enhance skin quality parameters. This case report included participants aged 43 and 58 years, both in good general health but exhibiting signs of skin atrophy or aging in the face or neck area. On Day 0, subjects underwent mesotherapy injections with non-crosslinked HA (18 mg/mL), supplemented with 0.01% calcium hydroxyapatite, glycine, and l-proline, administered at depths of 0.5 to 4 mm. On Day 7, a hydro-exfoliation and infrared treatment was performed, followed by a session with a non-ablative fractional 1470 nm laser. Skin quality improvements were visually assessed through comparative photographic analysis taken before treatment and four weeks post-procedure. The comparison revealed enhancements in wrinkle reduction, pore size, skin texture, and overall firmness. All treatments were well tolerated. In conclusion, the combination of non-crosslinked HA mesotherapy, hydro-exfoliation, infrared technology, and non-ablative fractional laser led to noticeable improvements in multiple skin quality parameters across a broad age range. While the study highlights the cosmetic benefits of this approach, further research is needed to comprehensively evaluate the long-term safety profile of these treatments, as the current methodology was not specifically designed for safety assessment.