Characterization of Adipose-Derived Mesenchymal Stem Cells from tissue harvested with the guided SEFFI technique and co-cultured with calcium hydroxyapatite

https://doi.org/10.56609/jac.v41i2.288

Authors

  • F. Melfa Aesthetic Physician Mediaging Clinic Center Palermo Milano Italy
  • A. Gennai General Surgeon Studio Gennai Bologna Italy
  • G. Carfagna Plastic Surgeon Carfagna Plastic Surgery Clinic Napoli Italy
  • B. Bovani General Surgeon Esculapio Clinic Perugia Republic of San Marino Italy
  • D. Piccolo Dermatologist Novea Skin Center Pescara Avezzano L’Aquila Italy
  • M. Colli Aesthetic Surgeon Milano Italy
  • M. Baldessin General Surgeon Studio MB Treviso Italy
  • D. Siragusa Aesthetic Physician Mediaging Clinic Center Palermo

Keywords:

Stromal vascular fraction, adipose-derived mesenchymal stem cells, autologous adipose tissue graft, calcium hydroxylapatite, antiaging therapy

Abstract

Increased interest in regenerative medicine in the last few years was registered. In parallel, a progressive increase in injectable calcium hydroxylapatite (CaHA) applications has been observed due to its applications in skin rejuvenation. This study combined micro fragmented fatty tissue (FAT) grafting with the CAHA filler procedure to observe the biological effect on FAT. Each sample of micro fragmented adipose tissue harvested using the guided  Superficial Enhanced Fluid Fat Injection (guided SEFFI) technique were collected from 5 patients. The 5 samples were divided into aliquots to create two different experimental conditions: FAT and FAT combined with CaHA (FAT + R). Afterwards, the cellularity of mesenchymal phenotype and the in vitro differentiation capacity in mesenchymal lineages were assessed in both experimental conditions, FAT and FAT+R. Despite low cellularity observed for FAT+R compared to FAT, isolated cells could grow and expand in culture in both experimental conditions, thus proving their proliferative ability. Cells were proved to differentiate towards mesenchymal lineages, expressing mesenchymal markers by flow cytometry analysis. Combining emulsified harvested tissue prepared with guided SEFFI technique with CaHA products can be exploited to counteract the loss of volume and skin ageing of the human face and body. This approach to regenerative aesthetic treatment is a promising treatment for facial antiaging therapy.

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Author Biography

F. Melfa, Aesthetic Physician Mediaging Clinic Center Palermo Milano Italy

Corresponding author:

Fabrizio Melfa, MD

Aesthetic Physician

Mediaging Clinic Center,

Palermo, Milano, Italy

Via Marchese di Villabianca 185

90141, Palermo 

Tel. +39 329 1972493

e-mail: dottormelfa@gmail.com

Published

2023-06-22

How to Cite

[1]
Melfa, F., Gennai, A., Carfagna, G., Bovani, B., Piccolo, D., Colli, M., Baldessin, M. and Siragusa, D. 2023. Characterization of Adipose-Derived Mesenchymal Stem Cells from tissue harvested with the guided SEFFI technique and co-cultured with calcium hydroxyapatite. Journal of Applied Cosmetology. 41, 2 (Jun. 2023), 4/19. DOI:https://doi.org/10.56609/jac.v41i2.288.