Polycaprolactone (PCL) as an Adipose-Derived Stem Cells (ADSCS) stimulator – the experimental trial

Abstract

This study investigates polycaprolactone (PCL, 25–50 μm microspheres in carboxymethylcellulose gel) as a stimulator of adipose-derived stem cells (ADSCs) for collagen production and tissue regeneration. Histological, immunohistochemical, and electron microscopy analyses in a rat model (n=25; 20 treated, 5 controls) over 21 days demonstrated significant ADSC activity (95% CD34 positivity), fibroblast proliferation (>50% increase), angiogenesis (55% CD31 positivity), controlled inflammation (low CD45), and organized collagen formation. Results indicate PCL’s superiority in regenerative medicine applications, offering minimal adverse reactions compared to traditional stimulators.