New Insights on Anti-Aging Activity of Chitin Nanofibril-Hyaluronan Block Copolymers Entrapping Active Ingredients: In Vitro and In Vivo Study
Keywords:
Chitin nanofibril, Hyaluronan, Block copolymer, Skin aging, In-office treatment, Intrinsic aging, Extrinsic aging, Rejuvenation treatmentAbstract
Background: Some observations suggest a similar molecular mechanism for genetic aging and photo-aging: the same proteins mediating cellular division and senescence, appear to mediate DNA damage, after UV irradiation or oxidative stress. Consequently collagen-fibers are disorganized or cross-linked and elastic fibers appear damaged, as well as redox signaling are amplified ,and chaperon proteins, assisting the folding of macromolecular structures as HSP47, are no more sufficiently synthesized. Thus, fine lines and wrinkles appear leading to formation of black spots, sagging, and loose skin.
Aims: It was designed to control in vitro and in vivo the antiaging activity of cosmetic formulations based on the use of Chitin nanofibril-Hyaluronan (CN-HA) block copolymeric nanoparticles, entrapping different active ingredients, to verify their effectiveness and safeness as rejuvenation treatment biologically active, capable to support and increase the in-office procedures of Plastic Surgeons and Dermatologists.
Methods: In vitro synthesis of collagen I, III, IV, and HSP47, as well as the release of IL-8 and Metalloproteinase-1, were controlled in fibroblast cultures by immunocytochemical methods, while the anti-collagenase activity and the relative citotoxicity were verified by colorimetric methods, on cultures of both keratinocytes and fibroblasts. In vivo. Skin hydration and TEWL were controlled by the 3C- system, while the whitening activitywas measured by the Chromameter C 300.
Results and Conclusions: According to our previous published studies, the obtained positive results confirm on one hand the capacity the block-copolymers CN-HA have to easily entrap and modulate the efficacy of different active ingredients ,increasing their delivery and effectiveness at level of the skin layers. On the other hand they seem to support the possibility for designing and formulating innovative anti-aging cosmetics, useful to optimize the in-office rejuvenation treatments of Plastic Surgeons and Dermatologists.
